Tecnologia_en2024 – Scudo Technology

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ANTIBACTERIAL EFFICACY

Analysis Methodology

Bacterial strains and exposure times

ISO 22196:2011, ASTM E 214.

Furthermore, the Department of Biomedical, Surgical and Dental Sciences – One Health Section of the University of Milan has created a specific method for livestock farming to simulate the work environment.

Bacterial strains and exposure times

The charge reduction test was performed using a 24-well cell culture plate (Ø16 mm, volume 1 mL).

.To allow for better handling of the elastomer sheets, a rectangular section measuring 5cm x 8cm was sampled and a disk of suitable dimensions was “punched” for insertion into the above-mentioned plate.

Using sterile forceps, each disk was placed on the bottom of a well and covered with 1 mL of each bacterial suspension.

We used six bacterial species (Staphylococcus aureus ATCC 6538, Streptococcusgalactiae ATCC 13813, Escherichia coli ATCC 25922, Salmonella ATCC 25928, Listeria ATCC 13932 and Legionella ATCC 33152) at two different concentrations: 10³ UFC/mL and 10⁴ UFC/mL.

The exposure times (time-points) used to have an indication of the possible dynamics of bacterial load reduction were:

– T0: contact with elastomers

– T1: 5 minutes post-contact (PT)

– T2: 30 minutes PT

– T3: 1 hour PT

– T5: 6 hours PT

– T6: 24 hours PT.

At each time point, an aliquot of microbial suspension (50 µL) was appropriately diluted in sterile saline (0.9% NaCl) and seeded (50 µL) on a plate with solid medium. After incubation at 37°C for 24 hours, the colonies were counted to obtain the starting load at each time point.

ANTIVIRAL EFFICACY

Analysis Methodology

Viruses and cells used and methods

The strain used is described in table 2.2.1, together with the host cell line. The original virus was thawed and adsorbed onto the cells at approximately 90% confluence as described in the UNI EN 12353:2021 standard, and then left to incubate at 35°C ± 1°C with 5% CO2 until a TID50/ml titre of approximately 6 Log was obtained.

MDCK cells are grown in MEM + 10% FBS, supplemented with L-glutamine, non-essential amino acids, penicillin and streptomycin (growth medium).

Cells at 80-90% confluence are used to perform the test, and the medium is supplemented with trypsin, BSA and HEPES buffer (maintenance medium).

Calculate antiviral activity

Evaluation of results

Viral infectivity is calculated with the following formula:

S = (10 x P)

where:
S: virus infectivity per ml for suspension of the test

P: average TCID50

For each sample, the infectivity of the recovered virus is determined, with the following:

N = (10 x TCID50/ml x V) / A

where:
N: infectivity of the recovered virus per cm2 of sample

V: volume in ml of the SCDLP broth added to the sample

A: surface area of the cover film in cm²

The following formula is used to calculate antiviral activity:

R = (Ut – Uο) – (At – U0) = Ut – At

Where:

R: antiviral activity

Uο: mean of the logarithm of TCID50/cm2 of the three untreated samples immediately after inoculation

Ut: mean of the logarithm of TCID50/cm2 of the three untreated samples after the contact time

At: mean of the logarithm of TCID50/cm2 of the three treated samples after the contact time

The antiviral efficacy of the treatment is evaluated based on the logarithmic reduction R.

Safety

SCUDO Elastomers allow you to guarantee high levels of hygiene thanks to their properties, eliminating viruses and bacteria from surfaces in a constant and complete way.

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Savings

Thanks to SCUDO Elastomers, applied in a variety of sectors, it is possible to reduce the risk of infections from viruses and bacteria, with a reduction in the use of antibiotics.